Porcine epidemic diarrhea virus 3CLpro causes apoptosis and collapse of mitochondrial membrane potential requiring its protease activity and signaling through MAVS.

Porcine epidemic diarrhea (PED) is a highly contagious and virulent intestinal infectious disease characterized by diarrhea, vomiting and dehydration. Although PEDV-induced apoptosis has been characterized in vitro and vivo, the functional proteins related to this event and the mechanism still need further research. Here, we firstly demonstrated that PEDV epidemic strain JS2013 could trigger apoptosis in a dose- and time-dependent manner. Then, PEDV 3CLpro was further identified as a crucial inducer of PEDV-triggered apoptosis. In addition, using site-directed mutagenesis to disrupt the protease activity of 3CLpro by His41 and Cys144 mutations, we found that 3CLpro-induced apoptosis and mitochondrial damage significantly reduced, suggesting that the protease activity of 3CLpro was essential for apoptosis and mitochondrial damage. Furthermore, PEDV 3CLpro could synergistically promote MAVS-mediated apoptosis and MAVS was involved in the signaling pathway of 3CLpro-induced apoptosis, but no direct interaction between PEDV 3CLpro and MAVS was detected by immunoprecipitation assays. Our findings provide important insights into the role of 3CLpro in the pathogenicity of PEDV.

Exploring essential travel during COVID-19 quarantine: Evidence from China.

The COVID-19 has created significant impacts on the economy and individual life around the world. Various countries and cities have adopted corresponding control measures to reduce transport activities and maintain social distance to combat the spread of COVID-19. In the circumstances, residents only maintained essential travel to ensure a normal and fundamental life. In order to explore the impacts of the epidemic and control measures on individually essential travel, we have collected 513 questionnaires between February and March 2020 in China to investigate the various characteristics of essential travel. Using a multivariate logistic regression model, we examine the major factors that potentially impact the mode choices of essential travel. Results show that various socioeconomic, transport supply, health concern and travel purpose have significantly influenced travel mode choices of essential travel. The concept of essential travel will, in the era of port-pandemic, have profound implications on transportation policy making, especially on how to improve the fundamental welfare of the disadvantaged population.